Deteksi Bakteri Penyebab CVPD pada Jeruk MenggunakanDNA Asal Tulang Daun

Jurnal Fitopatologi Indonesia

Nurjanah Nurjanah¹  Ariningsih Salji Endah¹  Nurmaida Nurmaida¹  Andi Prasetiawan¹  Ummu Salamah Rustiani² 

[1] Balai Besar Uji Standar Karantina Pertanian, Jakarta;Balai Uji Terap Teknik dan Metode Karantina , Pertanian, Bekasi;

关键词: Candidatus liberibacter asiaticus, karantina, metode deteksi CPVD;   

DOI  :  10.14692/jfi.11.3.79

来源: DOAJ

【 摘 要 】

A method for identification of the causal bacteria of citrus vein phloem degeneration (CVPD) based on polymerase chain reaction (PCR) technique using template DNA extracted from leaf midrib of citrus has been implemented routinely. The method has not been validated, therefore it is necessary to validate the method to confirm that the method fit for its intended use. Leaf samples showing chlorotic symptom was obtained from Bogor and Bekasi, West Java and used for test samples. These samples was differentiated into 2 groups, i.e. leaf midrib and leaf mesophyll. Validation test involved homogenicity, and reproducibility test; each test was replicated 2 times. The test showed that using leaf midrib gave better result for detection of bacteria causing CVPD disease than using leaf mesophyll. Therefore, this method is recommended as routine detection method for bacteria causing CVPD disease.

【 授权许可】

Unknown