Antimelanogenic Effect of an Oroxylum indicum Seed Extract by Suppression of MITF Expression through Activation of MAPK Signaling Protein

International Journal of Molecular Sciences

Yeong Ho Cha¹  Peijun Zhao¹  Hee-Jeong Choi¹  Sang-Han Lee¹  Hongyan An¹  Chi-Yeol Yoo¹  Md Badrul Alam²  Hyo-Hyun Kim³ 

[1] Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea;Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Korea;MR Innovation Co., Ltd., KNU Technopark, Daegu 41566, Korea;

关键词: antimelanogenic;    Oroxylum indicum;    mitogen-activated protein kinase (MAPK);    microphthalmia-associated transcription factor (MITF);   

DOI  :  10.3390/ijms19030760

来源: DOAJ

【 摘 要 】

In this study, the antimelanogenic effect of an ethyl acetate fraction of Oroxylum indicum Vent. seeds (OISEA) and its underlying mechanisms in melan-a cells were investigated. Antimelanogenesis activity was confirmed by assessing inhibition of tyrosinase activity and melanin content in the cells. Both transcriptional and translational expression of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase related protein-1 and 2 (TYRP-1 and TYRP-2), were also examined. The results depicted that pretreatment of OISEA significantly inhibits not only tyrosinase activity, but melanin production and intracellular tyrosinase activity. By repressing the expression of tyrosinase, TYRP-1, TYRP-2, and MITF, OISEA interrupted melanin production. Additionally, OISEA interfered with the phosphorylation of p38, extracellular signal-regulated kinase 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK), with the reversal of OISEA-induced melanogenesis inhibition after treatment with the specific inhibitors SB239063, U0126, and SP600125. Overall, these results suggest that OISEA can stimulate p38, ERK1/2, JNK phosphorylation, and subsequent suppression of melanin, leading to the inhibition of melanogenic enzymes and melanin production, possibly owing to the presence of polyphenolic compounds.

【 授权许可】

Unknown