Circulating oxidized LDL, increased in patients with acute myocardial infarction, is accompanied by heavily modified HDL[S]

Journal of Lipid Research

Naoko Sawada¹  Toshihiro Aiuchi¹  Takashi Obama¹  Rina Kato¹  Masaki Kikuchi¹  Yuji Hamazaki²  Shinji Koba²  Takashi Takaki³  Sanju Iwamoto⁴  Hiroyuki Itabe⁵ 

[1] Division of Biological Chemistry, Department of Pharmaceutical Sciences Showa University School of Pharmacy, Shinagawa-ku, Tokyo 142-8555, Japan;Division of Cardiology, Department of Medicine Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8555, Japan;Division of Electron Microscopy Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8555, Japan;Division of Physiology and Pathology, Department of Pharmacology, Toxicology, and Therapeutics Showa University School of Pharmacy, Shinagawa-ku, Tokyo 142-8555, Japan;To whom correspondence should be addressed;

关键词: atherosclerosis;    oxidized low density lipoprotein;    oxidized high density lipoprotein;    apolipoproteins;    proteomics;    lipidomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Oxidized LDL (oxLDL) is a known risk factor for atherogenesis. This study aimed to reveal structural features of oxLDL present in human circulation related to atherosclerosis. When LDL was fractionated on an anion-exchange column, in vivo-oxLDL, detected by the anti-oxidized PC (oxPC) mAb, was recovered in flow-through and electronegative LDL [LDL(−)] fractions. The amount of the electronegative in vivo-oxLDL, namely oxLDL in the LDL(−) fraction, present in patients with acute MI was 3-fold higher than that observed in healthy subjects. Surprisingly, the LDL(−) fraction contained apoA1 in addition to apoB, and HDL-sized particles were observed with transmission electron microscopy. In LDL(−) fractions, acrolein adducts were identified at all lysine residues in apoA1, with only a small number of acrolein-modified residues identified in apoB. The amount of oxPC adducts of apoB was higher in the LDL(−) than in the L1 fraction, as determined using Western blotting. The electronegative in vivo-oxLDL was immunologically purified from the LDL(−) fraction with an anti-oxPC mAb. The majority of PC species were not oxidized, whereas oxPC and lysoPC did not accumulate. Here, we propose that there are two types of in vivo-oxLDL in human circulating plasma and the electronegative in vivo-oxLDL accompanies oxidized HDL.

【 授权许可】

Unknown