Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics

Molecules

Gang Bai¹  Menglin Yan¹  Longfei Cong¹  Min Jiang¹  Shu Han²  Hong Du²  Kaixue Zhang³  Liu Zhang³  Haiying Bai³  Zhigang Yang³  Liyao Wang³  Lili Sun³  Guanhua Wei³ 

[1] College of Pharmacy, Nankai University, State Key Laboratory of Medicinal Chemical Biology, Tianjin 300350, China;School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China;School of Pharmacy, Lanzhou University, Lanzhou 730000, China;

关键词: angelica sinensis;    geoherb region;    chemical markers;    content;    uhplc-qtof-ms/ms;    metabolomics;    anti-inflammation;    calcium antagonists;   

DOI  :  10.3390/molecules24193536

来源: DOAJ

【 摘 要 】

This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.

【 授权许可】

Unknown