Hepatitis E Virus in Pork Production Chain in Czech Republic, Italy, and Spain, 2010

Emerging Infectious Diseases

关键词: Hepatitis E virus;    swine;    pork food chain;    molecular detection;    reverse transcriptase polymerase chain reaction;    zoonoses;   

DOI  :  10.3201/eid1808.111783

来源: DOAJ

【 摘 要 】

We evaluated the prevalence of hepatitis E virus (HEV) in the pork production chain in Czech Republic, Italy, and Spain during 2010. A total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for HEV by real-time quantitative PCR. Overall, HEV was higher in Italy (53%) and Spain (39%) than in Czech Republic (7.5%). HEV was detected most frequently in feces in Italy (41%) and Spain (39%) and in liver (5%) and meat (2.5%) in Czech Republic. Of 313 sausages sampled at processing and point of sale, HEV was detected only in Spain (6%). HEV sequencing confirmed only g3 HEV strains. Indicator virus (porcine adenovirus) was ubiquitous in fecal samples and absent in liver samples and was detected in 1 slaughterhouse meat sample. At point of sale, we found porcine adenovirus in sausages (1%–2%). The possible dissemination of HEV and other fecal viruses through pork production demands containment measures.

【 授权许可】

Unknown