Circadian rhythm of melatonin secretion and growth-related gene expression in the tiger puffer Takifugu rubripes

Fisheries and Aquatic Sciences

Sang-Woo Hur¹  Akihiro Takemura²  Yuki Takeuchi³  Sung-Pyo Hur⁴  Byeong-Hoon Kim⁵  Young-Don Lee⁵ 

[1] Aquafeed Research Center, National Institute of Fisheries Science (NIFS);Department of Chemistry, Biology and Marine Sciences, Faculty of Science, University of the Ryukyus;Department of Electrical Engineering and Bioscience, School of Advanced Science and Engineering, Waseda University;Jeju International Marine Science Research & Logistics Center, Korea Institute of Ocean Science & Technology;Marine Science Institute, Jeju National University;

关键词: Photoperiod;    Melatonin;    Somatostatin;    GHRH;    Tiger puffer;   

DOI  :  10.1186/s41240-017-0061-0

来源: DOAJ

【 摘 要 】

Abstract Somatostatin (SS) and growth hormone-releasing hormone (GHRH) are primary factors regulating growth hormone (GH) secretion in the pituitary. To date, it remains unknown how this rhythm is controlled endogenously, although there must be coordination of circadian manners. Melatonin was the main regulator in biological rhythms, and its secretion has fluctuation by photic information. But relationship between melatonin and growth-related genes (ghrh and ss) is unclear. We investigated circadian rhythms of melatonin secretion, ghrh and ss expressions, and correlation between melatonin with growth-related genes in tiger puffer Takifugu rubripes. The melatonin secretion showed nocturnal rhythms under light and dark (LD) conditions. In constant light (LL) condition, melatonin secretion has similar patterns with LD conditions. ss1 mRNA was high during scotophase under LD conditions. But ss1 rhythms disappeared in LL conditions. Ghrh appeared opposite expression compared with melatonin levels or ss1 expression under LD and LL. In the results of the melatonin injection, ghrh and ss1 showed no significant expression compared with control groups. These results suggested that melatonin and growth-related genes have daily or circadian rhythms in the tiger puffer. Further, we need to know mechanisms of each ss and ghrh gene regulation.

【 授权许可】

Unknown