期刊论文详细信息
Brain Sciences
Aneurysm-on-a-Chip: Setting Flow Parameters for Microfluidic Endothelial Cultures Based on Computational Fluid Dynamics Modeling of Intracranial Aneurysms
Anna Eigenbrodt1  Aisen Vivas1  Andries D. van der Meer1  Hieronymus D. Boogaarts2  Rene Aquarius2  Gabriela M. Ong3  Bernard J. Geurts3  Julia Mikhal3 
[1] Applied Stem Cell Technologies, University of Twente, 7522 NB Enschede, The Netherlands;Department of Neurosurgery, Radboud University Medical Center, 6525 XZ Nijmegen, The Netherlands;Multiscale Modeling and Simulation Group, Department of Applied Mathematics, University of Twente, 7522 NB Enschede, The Netherlands;
关键词: intracranial aneurysm;    aneurysm;    organ on a chip;    aneurysm on a chip;    endothelial cells;    computational fluid dynamics;   
DOI  :  10.3390/brainsci12050603
来源: DOAJ
【 摘 要 】

Intracranial aneurysms are pouch-like extrusions from the vessels at the base of the brain which can rupture and cause a subarachnoid hemorrhage. The pathophysiological mechanism of aneurysm formation is thought to be a consequence of blood flow (hemodynamic) induced changes on the endothelium. In this study, the results of a personalized aneurysm-on-a-chip model using patient-specific flow parameters and patient-specific cells are presented. CT imaging was used to calculate CFD parameters using an immersed boundary method. A microfluidic device either cultured with human umbilical vein endothelial cells (HUVECs) or human induced pluripotent stem cell-derived endothelial cells (hiPSC-EC) was used. Both types of endothelial cells were exposed for 24 h to either 0.03 Pa or 1.5 Pa shear stress, corresponding to regions of low shear and high shear in the computational aneurysm model, respectively. As a control, both cell types were also cultured under static conditions for 24 h as a control. Both HUVEC and hiPSC-EC cultures presented as confluent monolayers with no particular cell alignment in static or low shear conditions. Under high shear conditions HUVEC elongated and aligned in the direction of the flow. HiPSC-EC exhibited reduced cell numbers, monolayer gap formation and cells with aberrant, spread-out morphology. Future research should focus on hiPSC-EC stabilization to allow personalized intracranial aneurysm models.

【 授权许可】

Unknown   

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