Network analysis of nitrate-sensitive oral microbiome reveals interactions with cognitive function and cardiovascular health across dietary interventions

Redox Biology

Joanna E. L'Heureux¹  James Kelly²  Jonathan Fulford²  Jamie R. Blackwell²  Anni Vanhatalo²  Lee J. Wylie²  David W. Williams³  Andrew M. Jones⁴  Paul G. Winyard⁵  Mark van der Giezen⁶ 

[1]Corresponding author. St. Luke's Campus, University of Exeter, College Road, Exeter, EX1 1TE, Exeter, UK.

[2]College of Life and Environmental Sciences, University of Exeter, UK

[3]College of Medicine and Health, University of Exeter, UK

[4]Department of Chemistry, Bioscience and Environmental Engineering, University of Stavanger, Norway

[5]NIHR Exeter Clinical Research Facility, University of Exeter, UK

[6]School of Dentistry, Cardiff University, UK

关键词: Oral microbiome;    Nitric oxide;    Aging;   

DOI  :  

来源: DOAJ

【 摘 要 】

Many oral bacteria reduce inorganic nitrate, a natural part of a vegetable-rich diet, into nitrite that acts as a precursor to nitric oxide, a regulator of vascular tone and neurotransmission. Aging is hallmarked by reduced nitric oxide production with associated detriments to cardiovascular and cognitive function. This study applied a systems-level bacterial co-occurrence network analysis across 10-day dietary nitrate and placebo interventions to test the stability of relationships between physiological and cognitive traits and clusters of co-occurring oral bacteria in older people. Relative abundances of Proteobacteria increased, while Bacteroidetes, Firmicutes and Fusobacteria decreased after nitrate supplementation. Two distinct microbiome modules of co-occurring bacteria, that were sensitive to nitrate supplementation, showed stable relationships with cardiovascular (Rothia-Streptococcus) and cognitive (Neisseria-Haemophilus) indices of health across both dietary conditions. A microbiome module (Prevotella-Veillonella) that has been associated with pro-inflammatory metabolism was diminished after nitrate supplementation, including a decrease in relative abundance of pathogenic Clostridium difficile. These nitrate-sensitive oral microbiome modules are proposed as potential pre- and probiotic targets to ameliorate age-induced impairments in cardiovascular and cognitive health.

【 授权许可】

Unknown