The Effect of Seed-Borne Fungi and Epichloë Endophyte on Seed Germination and Biomass of Elymus sibiricus

Frontiers in Microbiology

Wayne R. Simpson¹  Zhi-Biao Nan²  Xiang Yao²  Xiu-Zhang Li²  Chun-Jie Li²  Qing Chai²  Mei-Ling Song² 

[1] AgResearch Grasslands Research Centre, Tennent Drive, Palmerston North, New Zealand;State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China;

关键词: Epichloë endophyte;    Elymus sibiricus;    seed-borne fungi;    seed germination;    Elymus tangutorum;    Achnatherum inebrians;   

DOI  :  10.3389/fmicb.2017.02488

来源: DOAJ

【 摘 要 】

The interactive effects of asexual Epichloë (formerly known as Neotyphodium) endophytes isolated from Hordeum brevisubulatum, Elymus tangutorum and Achnatherum inebrians, and seed-borne fungi on Elymus sibiricus seeds, were determined by an in vitro study using supernatants from liquid cultures of the endophyte strains. In an 8 week greenhouse study, the effects on the seedlings growth was measured. The in vitro study was carried out with the seed-borne fungi Alternaria alternata, Bipolaris sorokiniana, Fusarium avenaceum, and a Fusarium sp. isolated from E. sibiricus. Different concentrations and combinations of the liquid cultures of endophytic fungi enhanced the interim germination, germination rate, length of coleoptile and radicle, and seedling dry weight of E. sibiricus under stress from seed-borne fungi. In the greenhouse study, different concentrations of the supernatant of the endophytes from H. brevisubulatum and E. tangutorum but not A. inebrians, signficantly (P < 0.05) enhanced E. sibiricus seed germination. There was no significant (P > 0.05) increase of the tiller numbers after 2 weeks. However, later on, there were significant (P < 0.05) increases in tiller number (4–8 weeks), seedling height (2–8 weeks) and dry weight (2–8 weeks). The application of Epichloë endophyte culture supernatants was an effective strategy to improve seed germination and growth under greenhouse conditions.

【 授权许可】

Unknown