Raman Molecular Fingerprints of SARS‐CoV‐2 British Variant and the Concept of Raman Barcode

Advanced Science

Yuki Fujita¹  Giuseppe Pezzotti¹  Elia Marin¹  Wenliang Zhu¹  Francesco Boschetto¹  Narisato Kanamura²  Tetsuya Adachi²  Toshiro Yamamoto²  Osam Mazda³  Masaharu Shin‐Ya³  Eriko Ohgitani³  Ichiro Nishimura⁴ 

[1] Ceramic Physics Laboratory Kyoto Institute of Technology Sakyo‐ku, Matsugasaki Kyoto 606‐8585 Japan;Department of Dental Medicine Graduate School of Medical Science Kyoto Prefectural University of Medicine Kamigyo‐ku Kyoto 602‐8566 Japan;Department of Immunology Graduate School of Medical Science Kyoto Prefectural University of Medicine Kamigyo‐ku, 465 Kajii‐cho Kyoto 602‐8566 Japan;Division of Advanced Prosthodontics The Jane and Jerry Weintraub Center for Reconstructive Biotechnology UCLA School of Dentistry Los Angeles CA 90095 USA;

关键词: Raman;    barcode;    SARS‐CoV‐2;    variants;    fingerprints;   

DOI  :  10.1002/advs.202103287

来源: DOAJ

【 摘 要 】

Abstract The multiple mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) virus have created variants with structural differences in both their spike and nucleocapsid proteins. While the functional relevance of these mutations is under continuous scrutiny, current findings have documented their detrimental impact in terms of affinity with host receptors, antibody resistance, and diagnostic sensitivity. Raman spectra collected on two British variant sub‐types found in Japan (QK002 and QHN001) are compared with that of the original Japanese isolate (JPN/TY/WK‐521), and found bold vibrational differences. These included: i) fractions of sulfur‐containing amino acid rotamers, ii) hydrophobic interactions of tyrosine phenol ring, iii) apparent fractions of RNA purines and pyrimidines, and iv) protein secondary structures. Building upon molecular scale results and their statistical validations, the authors propose to represent virus variants with a barcode specially tailored on Raman spectrum. Raman spectroscopy enables fast identification of virus variants, while the Raman barcode facilitates electronic recordkeeping and translates molecular characteristics into information rapidly accessible by users.

【 授权许可】

Unknown