Human Neural Stem Cells Flown into Space Proliferate and Generate Young Neurons

Applied Sciences

Laurent Vergnes¹  MatthewJ. Schibler¹  LaurentA. Bentolila¹  Fathi Karouia²  Araceli Espinosa-Jeffrey³  Carlos Cepeda³  Nicholas Carpo³ 

[1] Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA;Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA;Departments of Psychiatry, UCLA, Los Angeles, CA 90095, USA;

关键词: neural stem cells;    pluripotency;    space flight;    microgravity;    proliferation;    neuronal specification;    neurons;    energetics;    glycolysis;   

DOI  :  10.3390/app9194042

来源: DOAJ

【 摘 要 】

Here we demonstrate that human neural stem cells (NSCs) proliferate while in space and they express specific NSC markers after being in space. NSCs displayed both higher oxygen consumption and glycolysis than ground controls. These cells also kept their ability to become young neurons. Electrophysiological recordings of space NSC-derived neurons showed immature cell membrane properties characterized by small capacitance and very high input resistance. Current injections elicited only an incipient action potential. No spontaneous synaptic events could be detected, suggesting their immature status even though most recorded cells displayed complex morphology and numerous cell processes. Ascertaining the origin of the NSCs’ increased energy requirement is of the essence in order to design effective measures to minimize health risks associated with long-duration human spaceflight missions.

【 授权许可】

Unknown