Effects of Space Flight on Expression of Key Proteins in Rice Leaves

Rice Science

Sun Yeqing¹  Cui Jie¹  Zhang Meng¹  Gao Xin¹  Zhang Yingchun²  Zeng Deyong³  Lu Weihong³  Shan Shan³  Yin Yishu³ 

[1] National and Local Joint Engineering Laboratory For Synthesis, Transformation and Separation of Extreme Environmental Nutrients, Harbin 150001, China;Dalian Maritime University, Institute of Environmental Systems Biology, Dalian 116026, China;School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China;

关键词: Differentially abundant protein;    Rice;    Proteomics;    Space flight;    Isobaric tags for relative and absolute quantitation;   

DOI  :  

来源: DOAJ

【 摘 要 】

As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth- and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage (TLS) and tillering stage (TS). In addition to comparing plant growth and reactive oxygen species (ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation (iTRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology.

【 授权许可】

Unknown