Simultaneous Determination of Albendazole and Its Three Metabolites in Pig and Poultry Muscle by Ultrahigh-Performance Liquid Chromatography-Fluorescence Detection

Foods

Yawen Guo¹  Tao Zhang¹  Zhaoyuan He¹  Zhixiang Diao¹  Yang Lu¹  Jinyuan Chen¹  Kaizhou Xie¹  Chun Xue¹  Lan Chen² 

[1] College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China;

关键词: ultrahigh-performance liquid chromatography-fluorescence detection (UPLC-FLD);    albendazole (ABZ) and its three metabolites;    pig muscle;    poultry muscle;   

DOI  :  10.3390/foods10102350

来源: DOAJ

【 摘 要 】

A fast, simple and efficient ultrahigh-performance liquid chromatography-fluorescence detection (UPLC-FLD) method for the determination of residues of albendazole (ABZ) and its three metabolites, albendazole sulfone (ABZ-SO₂), albendazole sulfoxide (ABZ-SO), and albendazole-2-aminosulfone (ABZ-2NH₂-SO₂), in pig and poultry muscle (chicken, duck and goose) was established. The samples were extracted with ethyl acetate, and the extracts were further subjected to cleanup by utilizing a series of liquid–liquid extraction (LLE) steps. Then, extracts were purified by OASIS^® PRiME hydrophilic-lipophilic balance (HLB) solid-phase extraction (SPE) cartridges (60 mg/3 mL). The target compounds were separated on an ACQUITY UPLC^® BEH C₁₈ (2.1 mm × 100 mm, 1.7 μm) chromatographic column, using a mobile phase composed of 31% acetonitrile and 69% aqueous solution (containing 0.2% formic acid and 0.05% triethylamine). The limits of detection (LODs) and limits of quantification (LOQs) of the four target compounds in pig and poultry muscle were 0.2–3.8 µg/kg and 1.0–10.9 µg/kg, respectively. The recoveries were all above 80.37% when the muscle samples were spiked with the four target compounds at the LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL, and 2.0 MRL levels. The intraday relative standard deviations (RSDs) were less than 5.11%, and the interday RSDs were less than 6.29%.

【 授权许可】

Unknown