A Pilot Cross-Sectional Study to Investigate the Biomarker Potential of Phosphorylated Neurofilament-H and Immune Mediators of Disability in Patients With 5 Year Relapsing-Remitting Multiple Sclerosis

Frontiers in Neurology

Macarena Filippo¹  María Inés Herrera¹  Rodolfo Alberto Kölliker-Frers³  Francisco Capani⁵  Santiago Perez Lloret⁶  Matilde Otero-Losada⁶  Julia Tau⁷  Andrés M. Villa⁸ 

[1] Centro de Investigaciones en Psicología y Psicopedagogía, Pontificia Universidad Católica Argentina (CIPP, UCA), Buenos Aires, Argentina;Departamento de Biología, Universidad John F. Kennedy, Buenos Aires, Argentina;Facultad de Farmacia y Bioquímica, Universidad Maimónides, Buenos Aires, Argentina;Facultad de Psicología, Pontificia Universidad Católica Argentina UCA, Buenos Aires, Argentina;Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago, Chile;Instituto de Investigaciones Cardiológicas, National Research Council, Universidad de Buenos Aires (ININCA, UBA-CONICET), Buenos Aires, Argentina;Laboratorio de Investigación Ocular, Departamento de Patología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina;Seccion de Neuroinmunología Clínica, Servicio de Neurología, Hospital José María Ramos íMejía, Buenos Aires, Argentina;

关键词: relapsing-remitting multiple sclerosis;    biomarker potential;    neurofilament heavy chain phosphoform;    cytokines;    disability progression;    expanded disability status scale;   

DOI  :  10.3389/fneur.2019.01046

来源: DOAJ

【 摘 要 】

Objective: To test the feasibility of conducting a full-scale project evaluating the potential value of the phosphorylated neurofilament H (pNF-H) and several cytokines as disability markers in relapsing-remitting multiple sclerosis (RRMS).Methods: Twenty-four patients with 5-year RRMS evolution and eleven healthy control subjects entered the study. None of the participants had an inflammatory systemic or metabolic disease. Disability progression was evaluated using the Expanded Disability Status Scale. Serum level of pNF-H, the anti-inflammatory cytokine transforming growth factor-β 1 (TGF-β1), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-17A (IL-17A), monocyte chemotactic protein-1 (MCP-1), and soluble intercellular cell-adhesion molecule 1 (sICAM-1) were quantified using enzyme-linked immunosorbent assay (ELISA).Results: The patients had higher serum level of TGF-β1, IL-6, sICAM-1, and pNF-H. Based on these findings, a sample of at least 49 controls and 89 recent-onset RRMS patients is required to find an at least 1-point between-group difference in pNF-H with a power of 80% and an α error = 0.05. The progression of the disease was correlated with the level of pNF-H (Spearman rho = 0.624, p = 0.006), but not with the cytokines'.Conclusions: The serum level of pNF-H, EDSS score-correlated, might stand for a potential biomarker of disability in RRMS reflecting progressive axonal damage and cumulative neurological deterioration. The novelty of these results warrants conducting a larger confirmatory trial.

【 授权许可】

Unknown