Genome and Phylogenetic Analysis of Genes Involved in the Immune System of Solea senegalensis – Potential Applications in Aquaculture

Frontiers in Genetics

Thomas Liehr¹  Aglaya García-Angulo²  Laureana Rebordinos²  María E. Rodríguez²  Manuel A. Merlo²  Silvia Portela-Bens² 

[1] Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany;Área de Genética, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Cádiz, Spain;

关键词: Solea senegalensis;    bacterial artificial chromosome;    immune system;    aquaculture;    syntenic conservation;   

DOI  :  10.3389/fgene.2019.00529

来源: DOAJ

【 摘 要 】

Global aquaculture production continues to increase rapidly. One of the most important species of marine fish currently cultivated in Southern Europe is Solea senegalensis, reaching more than 300 Tn in 2017. In the present work, 14 Bacterial Artificial Chromosome (BAC) clones containing candidate genes involved in the immune system (b2m, il10, tlr3, tap1, tnfα, tlr8, trim25, lysg, irf5, hmgb2, calr, trim16, and mx), were examined and compared with other species using multicolor Fluorescence in situ Hybridization (mFISH), massive sequencing and bioinformatic analysis to determine the genomic surroundings and syntenic chromosomal conservation of the genomic region contained in each BAC clone. The mFISH showed that the groups of genes hmgb2-trim25-irf5-b2m; tlr3-lysg; tnfα-tap1, and il10-mx-trim16 were co-localized on the same chromosomes. Synteny results suggested that the studied BACs are placed in a smaller number of chromosomes in S. senegalensis that in other species. Phylogenetic analyses suggested that the evolutionary rate of immune system genes studied is similar among the taxa studied, given that the clustering obtained was in accordance with the accepted phylogenetic relationships among these species. This study contributes to a better understanding of the structure and function of the immune system of the Senegalese sole, which is essential for the development of new technologies and products to improve fish health and productivity.

【 授权许可】

Unknown