Relationship between the expressions of PD-L1 and tumour-associated fibroblasts in gastric cancer

Artificial Cells, Nanomedicine, and Biotechnology

Linsong Mu¹  Wu Sui²  Yang Lin²  Wentao Yu²  Xiang Yu²  Hailong Su²  Chengkun Qin³ 

[1] Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China;Department of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, 264000, China;Department of Hepatological Surgery, Shandong Provincial Hospital affiliated to Shandong University, Jinan, 250021, China;

关键词: Gastric cancer;    TAF;    PD-1/PD-L1;    immune escape;   

DOI  :  10.1080/21691401.2019.1573741

来源: DOAJ

【 摘 要 】

Previous studies have focused on the changes of tumour cells in immune escape, and less is known about the effect of tumour microenvironment (TME) on immune escape. Tumour-associated fibroblasts (TAF) is an important part of the TME and has special physiological and biochemical characteristics, but the specific mechanism has not been clarified. In order to investigate the effect of TAF on the expression of PD-L1 in gastric cancer cells, gastric cancer cell lines MNK45, SGC7901 were non-contact co-culturing with TAF 1, 3 and 7 d via transwell. PD-L1 mRNA and protein expression were detected using qRT-PCR and FCM. Then, 95 cases of gastric cancer tissues were selected and evaluated PD-L1 and TAF expressions by immunohistochemical examination. The results showed that the mRNA and protein expression of PD-L1 in the experiment group were significantly higher than that in the control group. PD-L1 expression was associated with massive lymphocyte infiltration, diffuse/mixed histology and intratumoral TAFs in gastric cancers. In conclusion, TAFs promoted the growth in gastric cancer cell lines by increased the PD-L1 expression.

【 授权许可】

Unknown