Enzymatic Synthesis of Novel Vitexin Glucosides

Molecules

Tzi-Yuan Wang¹  Yun-Rong Zhang²  Shu-Yuan Lin²  Te-Sheng Chang²  Hsiou-Yu Ding³  Jiumn-Yih Wu⁴ 

[1] Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan;Department of Biological Sciences and Technology, National University of Tainan, Tainan 70005, Taiwan;Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan;Department of Food Science, National Quemoy University, Kinmen County 892, Taiwan;

关键词: glycoside hydrolase;    glycosyltransferase;    glycosylation;    vitexin;    glucoside;   

DOI  :  10.3390/molecules26206274

来源: DOAJ

【 摘 要 】

Vitexin is a C-glucoside flavone that exhibits a wide range of pharmaceutical activities. However, the poor solubility of vitexin limits its applications. To resolve this limitation, two glycoside hydrolases (GHs) and four glycosyltransferases (GTs) were assayed for glycosylation activity toward vitexin. The results showed that BtGT_16345 from the Bacillus thuringiensis GA A07 strain possessed the highest glycosylation activity, catalyzing the conversion of vitexin into new compounds, vitexin-4′-O-β-glucoside (1) and vitexin-5-O-β-glucoside (2), which showed greater aqueous solubility than vitexin. To our knowledge, this is the first report of vitexin glycosylation. Based on the multiple bioactivities of vitexin, the two highly soluble vitexin derivatives might have high potential for pharmacological usage in the future.

【 授权许可】

Unknown