Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays

Nanomaterials

Soraya López-Martín¹  Estefanía Lozano-Andrés¹  María Yáñez-Mó¹  María Matos²  Gemma Gutiérrez²  María Carmen Blanco-López³  Esther Serrano-Pertierra³  Pablo García-Manrique³ 

[1] Departamento de Biología Molecular, Universidad Autónoma de Madrid (UAM), 28049 Madrid, Spain;Department of Chemical Engineering and Environmental Technology, University of Oviedo, 33006 Asturias, Spain;Department of Physical and Analytical Chemistry, University of Oviedo, 33006 Asturias, Spain;

关键词: Artificial Extracellular Vesicles (EVs);    tetraspanins;    analytical standards;    immunoassays development;    biomimetic materials;   

DOI  :  10.3390/nano10050971

来源: DOAJ

【 摘 要 】

Quantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles with morphological and functional properties close to natural exosomes. This work presents the design, development and functional characteristics of fully artificial exosomes based on tetraspanin extracellular loops-coated niosomes, produced by bio-nanotechnology methods based on supra-molecular chemistry and recombinant protein technology. Mono- and double-functionalized particles with CD9/CD63 tetraspanins have been developed and characterized from a morphological and functional point of view. Produced bio-particles showed close similarities with natural entities in terms of physical properties. Their utility for bioanalysis is demonstrated by their detection and molecular-type discrimination by enzyme-linked immunosorbent assays (ELISAs), one of the most frequent bio-analytical method found in routine and research labs. The basic material based on streptavidin-coated niosomes allows the surface functionalization with any biotinylated protein or peptide, introducing versatility. Although promising results have been reported, further optimizations and deeper characterization will help this innovative biomaterial become a robust RM for validation and development of diagnostic tools for exosomes determination.

【 授权许可】

Unknown