A Perfusion Culture System for Assessing Bone Marrow Stromal Cell Differentiation on PLGA Scaffolds for Bone Repair

Frontiers in Bioengineering and Biotechnology

Mauro Alini¹  Jennifer J. Bara²  Alicia J. El Haj³  Martin J. Stoddart⁴  Katie Bardsley⁴  Caroline Moser⁵ 

[1] AO Research Institute Davos, Davos, Switzerland;Department of Orthopaedic Surgery, Washington University, St Louis, MO, United States;Healthcare Technology Institute, Institute of Translational Medicine, University of Birmingham, Birmingham, United Kingdom;Institute for Science and Technology in Medicine, Keele University, Keele, United Kingdom;Laboratory for Translational Nutritional Biology, Department of Health Sciences and Technologies, Institute of Food Nutrition and Health, ETH Zürich, Zürich, Switzerland;

关键词: perfusion bioreactor;    MSCs;    PLGA;    bone repair;    tissue engineering;   

DOI  :  10.3389/fbioe.2018.00161

来源: DOAJ

【 摘 要 】

Biomaterials development for bone repair is currently hindered by the lack of physiologically relevant in vitro testing systems. Here we describe the novel use of a bi-directional perfusion bioreactor to support the long term culture of human bone marrow stromal cells (BMSCs) differentiated on polylactic co-glycolic acid (PLGA). Primary human BMSCs were seeded onto porous PLGA scaffolds and cultured in static vs. perfusion culture conditions for 21 days in osteogenic vs. control media. PLGA scaffolds were osteoconductive, supporting a mature osteogenic phenotype as shown by the upregulation of Runx2 and the early osteocyte marker E11. Perfusion culture enhanced the expression of osteogenic genes Osteocalcin and Osteopontin. Extracellular matrix deposition and mineralisation were spatially regulated within PLGA scaffolds in a donor dependant manner. This, together with the observed upregulation of Collagen type X suggested an environment permissive for the study of differentiation pathways associated with both intramembranous and endochondral ossification routes of bone healing. This culture system offers a platform to assess BMSC behavior on candidate biomaterials under physiologically relevant conditions. Use of this system may improve our understanding of the environmental cues orchestrating BMSC differentiation and enable fine tuning of biomaterial design as we develop tissue-engineered strategies for bone regeneration.

【 授权许可】

Unknown