Construction of Biocompatible Hydrogel Scaffolds With a Long-Term Drug Release for Facilitating Cartilage Repair

Frontiers in Pharmacology

Xiong Xu¹  XiaoJie Yu²  Rui Chen³  GuoKe Tang⁴  Liang Zhu⁴  Yanbin Liu⁴  Wei Zhang⁵ 

[1] Department of Graduate, Hebei North University, Zhangjiakou, China;Department of Orthopedics, Hunan Aerospace Hospital, Changsha, China;Department of Orthopedics, Second Affiliated Hospital of Naval Medical University, Shanghai, China;Department of Orthopedics, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, China;Joint Surgery Department, Zhuzhou Central Hospital, Zhuzhou, China;

关键词: BMSCs;    cartilage regeneration;    hydrogel scaffold;    KGN;    long-term release;   

DOI  :  10.3389/fphar.2022.922032

来源: DOAJ

【 摘 要 】

In tissue engineering, hydrogel scaffolds allow various cells to be cultured and grown in vitro and then implanted to repair or replace the damaged areas. Here in this work, kartogenin (KGN), an effectively chondro-inductive non-protein bioactive drug molecule, was incorporated into a composite hydrogel comprising the positively charged chitosan (CS) and methacrylated gelatin (GelMA) polymers to fabricate appropriate microenvironments of bone marrow mesenchymal stem cells (BMSCs) for cartilage regeneration. Based on the combination of physical chain entanglements and chemical crosslinking effects, the resultant GelMA-CS@KGN composite hydrogels possessed favorable network pores and mechanical strength. In vitro cytotoxicity showed the excellent biocompatibility for facilitating the cell growth, adhesion, proliferation, and differentiation. The long-term sustainable KGN release from the hydrogel scaffolds in situ promoted the chondrogenic differentiation that can be employed as an alternative candidate for cartilage tissue regeneration.

【 授权许可】

Unknown