Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears

Advanced Science

Zhujun Feng¹  Yuefeng Hao²  Xing Yang²  Xiliang Shang³  Jun Chen³  Yunxia Li³  Yimeng Yang³  Jiangyu Cai³  Jingyi Lu³  Shiyi Chen³  Yuzhou Chen³  Jian Zhang³  Chen Chen⁴ 

[1] Department of Anatomy and Physiology School of Medicine Shanghai Jiao Tong University Shanghai 200025 China;Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Jiangsu 215500 China;Department of Sports Medicine Huashan Hospital Fudan University Shanghai 200040 China;Department of Sports Medicine Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University Shanghai 200233 China;

关键词: intra‐articular injection;    mesenchymal stem cells therapy;    PbS quantum dots;    second near‐infrared window;    supraspinatus tendon tear;   

DOI  :  10.1002/advs.201901018

来源: DOAJ

【 摘 要 】

Abstract Mesenchymal stem cells (MSCs) are capable of exerting strong therapeutic potential for the treatment of supraspinatus tendon tear. However, MSC therapy remains underutilized and perhaps underrated due to the limited evidence of dynamic visualization of cellular behavior in vivo. Here, second near‐infrared fluorescence imaging with biocompatible PbS quantum dots (QDs) provides a cellular migration map and information on the biodistribution and clearance processes of three densities of intra‐articularly injected, labeled MSCs to treat supraspinatus tendon tear in mice. Intra‐articular injection avoids entrapment of MSCs by filter organs and reduces the QD‐induced organ toxicity. Notably, the MSCs share a similar migration direction, but the moderate density group is somewhat more efficient, showing the longest residence time and highest cell retention rate around the footprint during the repair stage. Furthermore, quantitative kinetic investigation demonstrates that labeled MSCs are cleared by feces and urine. Histomorphometric analysis demonstrates that the moderate density group achieves maximum therapeutic effect and labeled MSCs do not induce any injury or inflammation to major organs, which suggests that administration of too many or few MSCs may decrease their effectiveness. Such an imaging approach provides spatiotemporal evidence for response to MSC therapy in vivo, facilitating the optimization of MSC therapy.

【 授权许可】

Unknown