Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors

Journal of Innovative Optical Health Sciences

Xi Li¹  Jia Yu²  Rongli Zhang²  Wei Zheng²  Hui Li² 

[1] Department of Gastroenterology, Peking University Shenzhen Hospital, Shen Zhen, Guangdong 518036, P. R. China;Research Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, P. R. China;

关键词: Two-photon excitation fluorescence microscopy;    fluorescence lifetime;    fluorescence spectrum;    digestive tract tumor diagnosis;    autofluorescence;   

DOI  :  10.1142/S179354581930009X

来源: DOAJ

【 摘 要 】

Digestive tract tumors account for 15% and 19.3% of the cancer incidence and deaths, respectively. Early detection of digestive tract tumors is crucial to the reduction of global cancer burden. Two-photon excitation fluorescence lifetime imaging microscopy (TP-FLIM) allows non-invasive, label-free, three-dimensional, high-resolution imaging of living tissues with not only histological but also biochemical characterization ability in both qualitative and quantitative way. Benefiting from these advantages, this technology is promising for clinical diagnosis of digestive tract tumors. In recent years, many efforts have been made in this field and some remarkable progress has been achieved. In this paper, we overview the recent progress of TP-FLIM-based researches on digestive tract tumor detection. Among them, our latest results on the gastric cancer and esophageal cancer are elaborately depicted. Finally, we outlook and discuss the potential advantages and challenges of TP-FLIM in future clinical applications.

【 授权许可】

Unknown