Differential Metabolic Reprogramming in Paenibacillus alvei-Primed Sorghum bicolor Seedlings in Response to Fusarium pseudograminearum Infection

Metabolites

IanA. Dubery¹  Fidele Tugizimana¹  PaulA. Steenkamp¹  Nico Labuschagne²  René Carlson² 

[1] Centre for Plant Metabolomics Research, Department of Biochemistry, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa;Department of Plant and Soil Sciences, Faculty of Plant Pathology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa;

关键词: crown rot;    Fusarium pseudograminearum;    induced systemic resistance;    LC-MS;    Paenibacillus alvei;    PGPR;    phytoalexin;    priming;    Sorghum bicolor;   

DOI  :  10.3390/metabo9070150

来源: DOAJ

【 摘 要 】

Metabolic changes in sorghum seedlings in response to Paenibacillus alvei (NAS-6G6)-induced systemic resistance against Fusarium pseudograminearum crown rot were investigated by means of untargeted ultra-high performance liquid chromatography-high definition mass spectrometry (UHPLC-HDMS). Treatment of seedlings with the plant growth-promoting rhizobacterium P. alvei at a concentration of 1 × 10⁸ colony forming units mL⁻¹ prior to inoculation with F. pseudograminearum lowered crown rot disease severity significantly at the highest inoculum dose of 1 × 10⁶ spores mL⁻¹. Intracellular metabolites were subsequently methanol-extracted from treated and untreated sorghum roots, stems and leaves at 1, 4 and 7 days post inoculation (d.p.i.) with F. pseudograminearum. The extracts were analysed on an UHPLC-HDMS platform, and the data chemometrically processed to determine metabolic profiles and signatures related to priming and induced resistance. Significant treatment-related differences in primary and secondary metabolism post inoculation with F. pseudograminearum were observed between P. alvei-primed versus naïve S. bicolor seedlings. The differential metabolic reprogramming in primed plants comprised of a quicker and/or enhanced upregulation of amino acid-, phytohormone-, phenylpropanoid-, flavonoid- and lipid metabolites in response to inoculation with F. pseudograminearum.

【 授权许可】

Unknown