Susceptibility Profile of Candida albicans Biofilms on Polyvinyl Chloride Endotracheal Tube to Antifungal Azoles

Journal of Pure and Applied Microbiology

Pauline Bridgette D. Salazar¹  Frederico Martin D. Alegre¹  Derick Erl P. Sumalapao²  Nina G. Gloriani³  Isidro C. Sia⁴  Nelson R. Villarante⁵ 

[1] Biology Department, College of Science, De La Salle University, Manila, Philippines. College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines. ;Department of Epidemiology and Biostatistics, College of Public Health, University of the Philippines Manila, Manila, Philippines. Biology Department, College of Science, De La Salle University, Manila, Philippines.;Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila, Philippines.;Department of Pharmacology and Toxicology, College of Medicine, University of the Philippines Manila, Manila, Philippines. Integrative Medicine for Alternative Healthcare Systems Philippines, Inc., Quezon City, Philippines.;Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Philippines.;

关键词: fluconazole;    fungal infections;    miconazole;    polymer-based material;    posaconazole;    standard disc diffusion method;    voriconazole;   

DOI  :  10.22207/JPAM.14.2.09

来源: DOAJ

【 摘 要 】

One of the most prevalent causative organisms in human fungal infections is Candida albicans. The extensive utility of medical devices such as endotracheal tube has resulted in the increased cases of fungal infections. The present study visualized Candida albicans biofilm on polyvinyl chloride endotracheal tube and described the susceptibility profile of the biofilm to antifungal azoles. Biofilm was examined using light and fluorescence microscopy. The susceptibility profile of planktonic and sessile cells with posaconazole, miconazole, voriconazole, and fluconazole was assessed employing the standard disc diffusion method. Planktonic C. albicans was susceptible to the identified antifungal azoles, whereas the sessile cells had varying susceptibility profiles. The biofilms remained susceptible to voriconazole and fluconazole, susceptible-dose dependent to posaconazole, and resistant to miconazole. Findings of the present study can provide relevant information on the resistance mechanism of fungal biofilms with azoles and in the design of new pharmacologic interventions intended as treatment of mycoses.

【 授权许可】

Unknown