TGF-β Activity Related to the Use of Collagen Membranes: In Vitro Bioassays

International Journal of Molecular Sciences

Layla Panahipour¹  Reinhard Gruber¹  Zahra Kargarpour¹  Bernadette Luza¹  Jung-Seok Lee² 

[1] Department of Oral Biology, School of Dentistry, Medical University of Vienna, 1090 Vienna, Austria;Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, 03722 Seoul, Korea;

关键词: TGF-β activity;    collagen membranes;    guided bone regeneration;    in vitro;   

DOI  :  10.3390/ijms21186636

来源: DOAJ

【 摘 要 】

Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration.

【 授权许可】

Unknown