Extracellular Vesicles from Different Pneumococcal Serotypes Are Internalized by Macrophages and Induce Host Immune Responses

Pathogens

José A. González-Reyes¹  Alfonso Olaya-Abril²  Manuel J. Rodríguez-Ortega²  Arturo Casadevall³  Rafael Prados-Rosales⁴  Liise-anne Pirofski⁵ 

[1] Departamento de Biología Celular, Fisiología e Inmunología, Campus de Excelencia Internacional CeiA3, Universidad de Córdoba, 14071 Córdoba, Spain;Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional CeiA3, Universidad de Córdoba, 14071 Córdoba, Spain;Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA;Department of Preventive Medicine and Public Health and Microbiology, Autonoma University of Madrid, 28029 Madrid, Spain;Montefiore Medical Center, Department of Medicine and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, NY 10467, USA;

关键词: host-pathogen interaction;    Streptococcus pneumoniae;    membrane vesicles;    immune response;   

DOI  :  10.3390/pathogens10121530

来源: DOAJ

【 摘 要 】

Bacterial extracellular vesicles are membranous ultrastructures released from the cell surface. They play important roles in the interaction between the host and the bacteria. In this work, we show how extracellular vesicles produced by four different serotypes of the important human pathogen, Streptococcus pneumoniae, are internalized by murine J774A.1 macrophages via fusion with the membrane of the host cells. We also evaluated the capacity of pneumococcal extracellular vesicles to elicit an immune response by macrophages. Macrophages treated with the vesicles underwent a serotype-dependent transient loss of viability, which was further reverted. The vesicles induced the production of proinflammatory cytokines, which was higher for serotype 1 and serotype 8-derived vesicles. These results demonstrate the biological activity of extracellular vesicles of clinically important pneumococcal serotypes.

【 授权许可】

Unknown