Morphological Manipulation of DNA Gel Microbeads with Biomolecular Stimuli

Nanomaterials

Benediktus Nixon Hapsianto¹  Marie Shinohara¹  Seiju Benner²  Yuto Ohno²  Yuuka Tanabe²  Yosuke Torii³  Kazuki Takada⁴  Shu Okumura⁵  Anthony Genot⁵  Alexandre Baccouche⁵  Nicolas Lobato-Dauzier⁵  Teruo Fujii⁵  Soo Hyeon Kim⁵ 

[1] Department of Bioengineering, The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japan;Department of Chemistry, School of Science, The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japan;Faculty of Agriculture, The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japan;Faculty of Pharmaceutical Sciences, The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-8654, Japan;LIMMS, CNRS-Institute of Industrial Science, UMI 2820, University of Tokyo, Tokyo 153-8505, Japan;

关键词: DNA nanotechnology;    droplet microfluidic device;    DNA hydrogels;    toehold-strands;    dynamic morphological transformation;   

DOI  :  10.3390/nano11020293

来源: DOAJ

【 摘 要 】

Hydrogels are essential in many fields ranging from tissue engineering and drug delivery to food sciences or cosmetics. Hydrogels that respond to specific biomolecular stimuli such as DNA, mRNA, miRNA and small molecules are highly desirable from the perspective of medical applications, however interfacing classical hydrogels with nucleic acids is still challenging. Here were demonstrate the generation of microbeads of DNA hydrogels with droplet microfluidic, and their morphological actuation with DNA strands. Using strand displacement and the specificity of DNA base pairing, we selectively dissolved gel beads, and reversibly changed their size on-the-fly with controlled swelling and shrinking. Lastly, we performed a complex computing primitive—A Winner-Takes-All competition between two populations of gel beads. Overall, these results show that strand responsive DNA gels have tantalizing potentials to enhance and expand traditional hydrogels, in particular for applications in sequencing and drug delivery.

【 授权许可】

Unknown