Assessment of Antifungal Efficacy and Release Behavior of Fungicide-Loaded Chitosan-Carrageenan Nanoparticles against Phytopathogenic Fungi

Polymers

Ravinder Kumar¹  Joginder Singh Duhan¹  Pardeep Kumar Sadh¹  Joanna Klepacka²  Prince Chawla³  Seweryn Malawski⁴  Anil Kumar Poonia⁵  Agnieszka Najda⁶  Balvinder Kumar⁷ 

[1] Department of Biotechnology, Chaudhary Devi Lal University, Sirsa 125055, Haryana, India;Department of Commodity Science and Food Analysis, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, 2 Oczapowskiego Street, 10-719 Olsztyn, Poland;Department of Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara 144411, Punjab, India;Department of Landscape Architecture, University of Life Science in Lublin, 28 Głęboka Street, 20-400 Lublin, Poland;Department of Molecular Biology, Biotechnology & Bioinformatics, CCS HAU, Hisar 125004, Haryana, India;Department of Vegetable and Herbal Crops, University of Life Science in Lublin, 50A Doświadczalna Street, 20-280 Lublin, Poland;ICAR-National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India;

关键词: antifungal;    nanocomposite;    chitosan;    bioactivity;    polysaccharide;    binary blend;   

DOI  :  10.3390/polym14010041

来源: DOAJ

【 摘 要 】

Biopolymeric Chitosan-Carrageenan nanocomposites 66.6–231.82 nm in size containing the chemical fungicide mancozeb (nano CSCRG-M) were synthesized following a green chemistry approach. The physicochemical study of nanoparticles (NPs) was accomplished using a particle size analyzer, SEM and FTIR. TEM exhibited clover leaf-shaped nanoparticles (248.23 nm) with mancozeb on the inside and entrapped outside. Differential scanning calorimetry and TGA thermogravimetry exhibited the thermal behaviour of the nanoform. Nano CSCRG-1.5 at 1.5 ppm exhibited 83.1% inhibition against Alternaria solani in an in vitro study and performed as well as mancozeb (84.6%). Complete inhibition was exhibited in Sclerotinia sclerotiorum at 1.0 and 1.5 ppm with the nanoformulation. The in vivo disease control efficacy of mancozeb-loaded nanoparticles against A. solani in pathogenized plants was found to be relatively higher (79.4 ± 1.7) than that of commercial fungicide (76 ± 1.1%) in pot conditions. Nanomancozeb showed superior efficacy for plant growth parameters, such as germination percentage, root–shoot ratio and dry biomass. The nanoformulation showed higher cell viability compared to mancozeb in Vero cell cultures at 0.25 and 0.50 mg/mL in the resazurin assay. CSCRG-0.5 showed slow-release behavior up to 10 h. Thus, these green nano-based approaches may help combat soil and water pollution caused by harmful chemical pesticides.

【 授权许可】

Unknown