Oral and Intravenous Iron Therapy Differentially Alter the On- and Off-Tumor Microbiota in Anemic Colorectal Cancer Patients

Cancers

Jonathan Segal¹  EdwardA. Dickson²  AustinG. Acheson²  Aditi Kumar³  Helen Steed³  MatthewJ. Brookes³  Oliver Phipps³  HafidO. Al-Hassi³  MohammedN. Quraishi⁴  AndrewD. Beggs⁴ 

[1] Department of Gastroenterology, St. Mark’s Hospital, Harrow HA1 3UJ, UK;NIHR Biomedical Research Centre in Gastrointestinal and Liver Diseases, Nottingham University Hospitals NHS Trust, Nottingham NG7 2UH, UK;Research Institute in Healthcare Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UK;Surgical Research Laboratory, Institute of Cancer & Genomic Science, University of Birmingham, Birmingham B15 2TQ, UK;

关键词: iron supplementation;    iron deficiency;    anemia;    gut microbiome;    16S rRNA;    tumor microbiota;   

DOI  :  10.3390/cancers13061341

来源: DOAJ

【 摘 要 】

Iron deficiency anemia is a common complication of colorectal cancer and may require iron therapy. Oral iron can increase the iron available to gut bacteria and may alter the colonic microbiota. We performed an intervention study to compare oral and intravenous iron therapy on the colonic tumor-associated (on-tumor) and paired non-tumor-associated adjacent (off-tumor) microbiota. Anemic patients with colorectal adenocarcinoma received either oral ferrous sulphate (n = 16) or intravenous ferric carboxymaltose (n = 24). On- and off-tumor biopsies were obtained post-surgery and microbial profiling was performed using 16S ribosomal RNA analysis. Off-tumor α- and β-diversity were significantly different between iron treatment groups. No differences in on-tumor diversity were observed. Off-tumor microbiota of oral iron-treated patients showed higher abundances of the orders Clostridiales, Cytophagales, and Anaeroplasmatales compared to intravenous iron-treated patients. The on-tumor microbiota was enriched with the orders Lactobacillales and Alteromonadales in the oral and intravenous iron groups, respectively. The on- and off-tumor microbiota associated with intravenous iron-treated patients infers increased abundances of enzymes involved in iron sequestration and anti-inflammatory/oncogenic metabolite production, compared to oral iron-treated patients. Collectively, this suggests that intravenous iron may be a more appropriate therapy to limit adverse microbial outcomes compared to oral iron.

【 授权许可】

Unknown