Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their Response to Porcine Reproductive and Respiratory Syndrome Virus

Frontiers in Immunology

Nicolas Bertho¹  Elisa Crisci³  Mickael Bourge⁴  Maxence Frétaud⁵  Christelle Langevin⁵  Alexis Pleau⁶  Olivier Boulesteix⁶  Jérémy Pezant⁶  Thibaut Larcher⁷  Elisabetta Giuffra⁸  Patricia Renson⁹  Olivier Bourry⁹  Stéphane Rault^1,10  Elise Bordet^1,10  Isabelle Schwartz-Cornil^1,10  Edwige Bouguyon^1,10 

[1] 0BIOEPAR, INRA, Oniris, Nantes, France;Anses, Laboratoire de Ploufragan-Plouzané-Niort, Unité Virologie et Immunologie Porcines, Zoopôle, BP53, Ploufragan, France;Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States;I2BC, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France;INRA, EMERG'IN- Plateforme d'Infectiologie Expérimentale IERP- Domaine de Vilvert, Jouy-en-Josas, France;INRA, UE1277, Plate-Forme d'Infectiologie Expérimentale, PFIE, Nouzilly, France;INRA, UMR 703 APEX, Oniris, Nantes, France;UMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France;Université Bretagne Loire, Cité Internationale, Rennes, France;VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France;

关键词: swine;    lymph node;    PRRSV;    macrophage;    B cell;    centrocyte;   

DOI  :  10.3389/fimmu.2019.00953

来源: DOAJ

【 摘 要 】

Swine lymph nodes (LN) present an inverted structure compared to mouse and human, with the afferent lymph diffusing from the center to the periphery. This structure, also observed in close and distant species such as dolphins, hippopotamus, rhinoceros, and elephants, is poorly described, nor are the LN macrophage populations and their relationship with B cell follicles. B cell maturation occurs mainly in LN B cell follicles with the help of LN macrophage populations endowed with different antigen delivery capacities. We identified three macrophage populations that we localized in the inverted LN spatial organization. This allowed us to ascribe porcine LN MΦ to their murine counterparts: subcapsular sinus MΦ, medullary cord MΦ and medullary sinus MΦ. We identified the different intra and extrafollicular stages of LN B cells maturation and explored the interaction of MΦ, drained antigen and follicular B cells. The porcine reproductive and respiratory syndrome virus (PRRSV) is a major porcine pathogen that infects tissue macrophages (MΦ). PRRSV is persistent in the secondary lymphoid tissues and induces a delay in neutralizing antibodies appearance. We observed PRRSV interaction with two LN MΦ populations, of which one interacts closely with centroblasts. We observed BCL6 up-regulation in centroblast upon PRRSV infection, leading to new hypothesis on PRRSV inhibition of B cell maturation. This seminal study of porcine LN will permit fruitful comparison with murine and human LN for a better understanding of normal and inverted LN development and functioning.

【 授权许可】

Unknown