Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy

Biosensors

Takumi Sannomiya¹  Andreas Frutiger²  Morteza Aramesh²  Raphael F. Tiefenauer²  Lukas Schmidheini²  Volker Gatterdam² 

[1] Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Yokohama 226-8503, Japan;Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zürich, 8092 Zürich, Switzerland;

关键词: blinking nanodiamonds;    gold nanoparticles;    plasmonic coupling;    multiphoton excitation;    nanoscopy;   

DOI  :  10.3390/bios12030148

来源: DOAJ

【 摘 要 】

Nanodiamonds have emerged as promising agents for sensing and imaging due to their exceptional photostability and sensitivity to the local nanoscale environment. Here, we introduce a hybrid system composed of a nanodiamond containing nitrogen-vacancy center that is paired to a gold nanoparticle via DNA hybridization. Using multiphoton optical studies, we demonstrate that the harmonic mode emission generated in gold nanoparticles induces a coupled fluorescence emission in nanodiamonds. We show that the flickering of harmonic emission in gold nanoparticles directly influences the nanodiamonds’ emissions, resulting in stochastic blinking. By utilizing the stochastic emission fluctuations, we present a proof-of-principle experiment to demonstrate the potential application of the hybrid system for super-resolution microscopy. The introduced system may find applications in intracellular biosensing and bioimaging due to the DNA-based coupling mechanism and also the attractive characteristics of harmonic generation, such as low power, low background and tissue transparency.

【 授权许可】

Unknown