Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets

Frontiers in Microbiology

Xiujuan Yuan¹  Yichao Jiang²  Huaibin Kang³  Peng Fei³  Tongxiang Yang³  Junliang Chen³  Ziyuan Wang³  Yan Jiang⁴  Stephen J. Forsythe⁵ 

[1] Anda Department of Animal Husbandry and Veterinary, Anda, China;Changbai Mountains Food and Drug Inspection Testing Center, Baishan, China;College of Food and Biological Engineering, Henan University of Science and Technology, Luoyang, China;Department of Market Supervision and Management, MuLing Food Inspection Testing Center, Mudanjiang, China;Foodmicrobe.com, Nottingham, United Kingdom;

关键词: C. sakazakii;    prevalence;    genotyping;    antibiotic susceptibility;    powdered infant formula (PIF);   

DOI  :  10.3389/fmicb.2017.02026

来源: DOAJ

【 摘 要 】

Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii isolates from PIF purchased from Chinese retail markets. Two thousand and twenty PIF samples were collected from different institutions. Fifty-six C. sakazakii strains were isolated, and identified using fusA sequencing analysis, giving a contamination rate of 2.8%. Multilocus sequence typing (MLST) was more discriminatory than other genotyping methods. The C. sakazakii isolates were divided into 14 sequence types (STs) by MLST, compared with only seven clusters by ompA and rpoB sequence analysis, and four C. sakazakii serotypes by PCR-based O-antigen serotyping. C. sakazakii ST4 (19/56, 33.9%), ST1 (12/56, 21.4%), and ST64 (11/56, 16.1%) were the dominant sequence types isolated. C. sakazakii serotype O2 (34/56, 60.7%) was the primary serotype, along with ompA6 and rpoB1 as the main allele profiles, respectively. Antibiotic susceptibility testing indicated that all C. sakazakii isolates were susceptible to ampicillin-sulbactam, cefotaxime, ciprofloxacin, meropenem, tetracycline, piperacillin-tazobactam, and trimethoprim-sulfamethoxazole. The majority of C. sakazakii strains were susceptible to chloramphenicol and gentamicin (87.5 and 92.9%, respectively). In contrast, 55.4% C. sakazakii strains were resistant to cephalothin. In conclusion, this large-scale study revealed the prevalence and characteristics of C. sakazakii from PIF in Chinese retail markets, demonstrating a potential risk for neonates and infants, and provide a guided to effective control the contamination of C. sakazakii in production process.

【 授权许可】

Unknown