DDR1 Affects Metabolic Reprogramming in Breast Cancer Cells by Cross-Talking to the Insulin/IGF System

Biomolecules

Andrea Morrione¹  Rosamaria Lappano²  Marcello Maggiolini²  Marika Giuliano³  Maria Luisa Nicolosi³  Antonino Belfiore³  Małgorzata Anna Marć³  Veronica Vella³  Maria Giovanna Majorana³  Maria Grazia Muoio³  Ernestina Marianna De Francesco³ 

[1] Center for Biotechnology, Department of Biology, Sbarro Institute for Cancer Research and Molecular Medicine, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA;Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, Italy;Unit of Endocrinology, Department of Clinical and Experimental Medicine, University of Catania, Garibaldi-Nesima Hospital, 95122 Catania, Italy;

关键词: discoidin domain receptor 1 (DDR1);    metabolic reprogramming;    tumor metabolism;    insulin;    hyperinsulinemia;    insulin receptor (IR);   

DOI  :  10.3390/biom11070926

来源: DOAJ

【 摘 要 】

The insulin receptor isoform A (IR-A), a dual receptor for insulin and IGF2, plays a role in breast cancer (BC) progression and metabolic reprogramming. Notably, discoidin domain receptor 1 (DDR1), a collagen receptor often dysregulated in cancer, is involved in a functional crosstalk and feed forward loop with both the IR-A and the insulin like growth factor receptor 1 (IGF1R). Here, we aimed at investigating whether DDR1 might affect BC cell metabolism by modulating the IGF1R and/or the IR. To this aim, we generated MCF7 BC cells engineered to stably overexpress either IGF2 (MCF7/IGF2) or the IR-A (MCF7/IR-A). In both cell models, we observed that DDR1 silencing induced a significant decrease of total ATP production, particularly affecting the rate of mitochondrial ATP production. We also observed the downregulation of key molecules implicated in both glycolysis and oxidative phosphorylation. These metabolic changes were not modulated by DDR1 binding to collagen and occurred in part in the absence of IR/IGF1R phosphorylation. DDR1 silencing was ineffective in MCF7 knocked out for DDR1. Taken together, these results indicate that DDR1, acting in part independently of IR/IGF1R stimulation, might work as a novel regulator of BC metabolism and should be considered as putative target for therapy in BC.

【 授权许可】

Unknown