Isolation of CHS Gene from Brunfelsia acuminata Flowers and Its Regulation in Anthocyanin Biosysthesis

Molecules

Yu-Ting Cao¹  Dong-Liang Qiu¹  Min Li¹  Teng-Fei Pan¹  Muhammad Irshad¹  Si-Rui Ye¹ 

[1] College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

关键词: Brunfelsia acuminata;    flower color;    anthocyanin biosynthesis;    chalcone synthase (CHS);    RT-qPCR;   

DOI  :  10.3390/molecules22010044

来源: DOAJ

【 摘 要 】

Chalcone synthase gene (BaCHS) from Brunfelsia acuminata flowers was isolated using RT-PCR and RACE. The coding region of the gene is 1425-bp with an open reading frame of 1170-bp, 73-bp 5′UTR, and 172-bp 3′UTR. Its deduced protein does not have a signal peptide but does contain a cond_enzyme superfamily domain, and consists of 389 amino acids with a predicted molecular mass of 42,699 Da and a pI of 6.57. The deduced amino acid sequence of BaCHS shares 90%, 88%, 85%, 84% and 79% identity with CHS from Petunia hybrida, Nicotiana tabacum, Solanum lycopersicum, Capsicum annuum and Camellia sinensis, respectively. The striking color change from dark purple to light purple and ultimately lead to pure white resulted from a decline in anthocyanin content of the petals and was preceded by a decrease in the expression of BaCHS. Its gene expression was positively correlated with the contents of anthocyanin (p ≤ 0.01).

【 授权许可】

Unknown