Genotypic analysis of secreted aspartyl proteinases in vaginal Candida albicans isolates

Jornal Brasileiro de Patologia e Medicina Laboratorial

关键词: vulvovaginal candidiasis;    Candida albicans;    virulence factors;   

DOI  :  10.5935/1676-2444.20180006

来源: DOAJ

【 摘 要 】

ABSTRACTIntroduction: Candida albicans is the most common etiologic agent of fungal vaginitis. These yeasts produce secreted aspartyl proteinases encoded by a family of 10 genes (SAP1-10).Objective: The purpose of this study was to analyze the presence of genes SAP1-7 in vulvovaginal C. albicans.Materials and method: The study included 26 C. albicans vaginal isolates. Detection of aspartyl proteases genes (SAP1-7) was performed by polymerase chain reaction (PCR).Results: The most frequent gene in C. albicans isolated from colonization was SAP6 (93.33%), and from infection, SAP7 (100%). We observed a statistical difference (p = 0.049) in SAP1 gene frequency between isolates from vulvovaginal colonization and infection.Conclusion: High frequency of SAP genes was observed in vulvovaginal C. albicans. The results suggest SAP1 participation in vulvovaginal candidiasis infection.

【 授权许可】

Unknown