A Synthetic Derivative of Antimicrobial Peptide Holothuroidin 2 from Mediterranean Sea Cucumber (Holothuria tubulosa) in the Control of Listeria monocytogenes

Marine Drugs

Alessandra Magistrato¹  Angelo Spinello¹  Barbara Parrino²  MariaGrazia Cusimano²  Vincenzo Arizza²  Domenico Schillaci²  Stella Cascioferro²  Giampaolo Barone²  Maria Vitale³ 

[1] CNR-IOM-Democritos c/o International School for Advanced Studies (SISSA), Via Bonomea 265, 34136 Trieste, Italy;Dipartimento di Scienze Biologiche, Chimiche e Farmaceutiche, Università di Palermo, Via Archirafi 32, 90123 Palermo, Italy;Istituto Zooprofilattico della Sicilia, Via Gino Marinuzzi, 3, 90129 Palermo, Italy;

关键词: antimicrobial peptides;    Holothuria tubulosa;    foodborne pathogens;    Listeria monocytogenes;    biofilm;   

DOI  :  10.3390/md17030159

来源: DOAJ

【 摘 要 】

Due to the limited number of available antibiotics, antimicrobial peptides (AMPs) are considered antimicrobial candidates to fight difficult-to-treat infections such as those associated with biofilms. Marine environments are precious sources of AMPs, as shown by the recent discovery of antibiofilm properties of Holothuroidin 2 (H2), an AMP produced by the Mediterranean sea cucumber Holothuria tubulosa. In this study, we considered the properties of a new H2 derivative, named H2d, and we tested it against seven strains of the dangerous foodborne pathogen Listeria monocytogenes. This peptide was more active than H2 in inhibiting the growth of planktonic L. monocytogenes and was able to interfere with biofilm formation at sub-minimum inhibitory concentrations (MICs). Atomic-level molecular dynamics (MD) simulations revealed insights related to the enhanced inhibitory activity of H2d, showing that the peptide is characterized by a more defined tertiary structure with respect to its ancestor. This allows the peptide to better exhibit an amphipathic character, which is an essential requirement for the interaction with cell membranes, similarly to other AMPs. Altogether, these results support the potential use of our synthetic peptide, H2d, as a template for the development of novel AMP-based drugs able to fight foodborne that are resistant to conventional antibiotics.

【 授权许可】

Unknown