Effect of Three Dimensional Culture of Porcine Endometrial Cells on Their Plasminogen Activity and Pre-implantation Embryo Development after Co-culture

Journal of Animal Reproduction and Biotechnology

Min-Gyeong Kim¹  Yong HwangBo²  Hye-Jin Cha²  Su-Ji Kim²  Choon-Keun Park²  Sang-Hee Lee²  Boo-Keun Yang²  Hee-Tae Cheong³ 

[1] College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, K;College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, Korea;College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Ko;

关键词: 3-dimensional culture system;    embryo;    co-culture;    plasminogen activator;    implantation;   

DOI  :  10.12750/JET.2014.29.2.207

来源: DOAJ

【 摘 要 】

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in 2.5×105 cells/ml and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of IL-1β (0.1, 1, 10 and 100 ng/ml) were higher than without IL-1β, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

【 授权许可】

Unknown