Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte

Medical Laboratory Technology Journal

Dina Trikumalasari¹  Miftahul Mushlih¹  Rohman Dijaya¹  Dian Delta¹  Siska Aprilia¹  Livia Nur Azzah¹  Qilmia Fanani¹  Andika Aliviameita¹  Evi Rinata¹  Puspitasari Puspitasari² 

[1] ;universitas muhammadiyah sidoarjo;

关键词: type ii diabetes mellitus;    genom quality;    white blood cell (wbc);    temperature;    storage duration;   

DOI  :  10.31964/mltj.v5i2.229

来源: DOAJ

【 摘 要 】

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples.DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000).The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p

【 授权许可】

Unknown