期刊论文详细信息
Agronomy
Molecular Assisted Selection for Pollination-Constant and Non-Astringent Type without Male Flowers in Spanish Germplasm for Persimmon Breeding
Maríadel Mar Naval1  Manuel Blasco1  Francisco Gil-Muñoz2  MaríaLuisa Badenes2 
[1] CANSO, Mestre Serrano, 1, 46250 L’Alcúdia, Valencia, Spain;Instituto Valenciano de Investigaciones Agrarias, CV 315 km 10,5., 46113 Moncada, Valencia, Spain;
关键词: persimmon;    sex determination;    fruit astringency;    molecular markers;   
DOI  :  10.3390/agronomy10081172
来源: DOAJ
【 摘 要 】

Persimmon (Diospyros kaki Thunb) species is a hexaploid genotype that has a morphologically polygamous gyonodioecious sexual system. D. kaki bears unisexual flowers. The presence of male flowers resulted in the presence of seeds in the varieties. The fruits of persimmon are classified according to their astringency and the pollination events that produced seeds and modify the levels of astringency in the fruit. The presence of seeds in astringent varieties as pollination variant astringent (PVA), pollination variant non-astringent (PVNA) and pollination constant astringent (PCA) resulted in fruits not marketable. Molecular markers that allow selection of the varieties according to the type of flowers at the plantlet stage would allow selection of seedless varieties. In this study, a marker developed in D. lotus by bulk segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) markers, named DlSx-AF4, has been validated in a germplasm collection of persimmon, results obtained agree with the phenotype data. A second important trait in persimmon is the presence of astringency in ripened fruits. Fruits non-astringent at the ripen stage named pollination constant non-astringent (PCNA) are the objective of many breeding programs as they do not need removal of the astringency by a postharvest treatment. Astringency in the hexaploid persimmon is a dominant trait. The presence of at least one astringent allele confers astringency to the fruit. In this paper we checked the marker developed linked to the AST gene. Our goal has been to validate both markers in germplasm from different origins and to test the usefulness in a breeding program.

【 授权许可】

Unknown   

  文献评价指标  
  下载次数:0次 浏览次数:1次