The Roles of GmERF135 in Improving Salt Tolerance and Decreasing ABA Sensitivity in Soybean

Frontiers in Plant Science

Zhao-Shi Xu¹  Jia-Cheng Zheng¹  Jin-Hao Lan²  Jian Ma³  Yan-Xia Wang⁴  You-Zhi Ma⁵  Meng-Jie Zhao⁵  Li-Juan Yin⁵  Jin-Dong Fu⁵  Ming Chen⁵ 

[1] College of Agriculture, Anhui University of Science and Technology, Fengyang County, China;College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, China;Department of Agronomy, Jilin Agricultural University, Changchun, China;Hebei Academy of Agriculture and Forestry Sciences, Research Center of Wheat Engineering Technology of Hebei, Shijiazhuang, China;Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, China;

关键词: ABA;    ethylene-responsive factor;    hypocotyl elongation;    root growth;    response mechanism;    salt tolerance;   

DOI  :  10.3389/fpls.2019.00940

来源: DOAJ

【 摘 要 】

Abscisic acid (ABA) mediates various abiotic stress responses, and ethylene responsive factors (ERFs) play vital role in resisting stresses, but the interaction of these molecular mechanisms remains elusive. In this study, we identified an ABA-induced soybean ERF gene GmERF135 that was highly up-regulated by ethylene (ET), drought, salt, and low temperature treatments. Subcellular localization assay showed that the GmERF135 protein was targeted to the nucleus. Promoter cis-acting elements analysis suggested that numerous potential stress responsive cis-elements were distributed in the promoter region of GmERF135, including ABA-, light-, ET-, gibberellin (GA)-, and methyl jasmonate (MeJA)-responsive elements. Overexpression of GmERF135 in Arabidopsis enhanced tolerance to drought and salt conditions. In addition, GmERF135 promoted the growth of transgenic hairy roots under salt and exogenous ABA conditions. These results suggest that soybean GmERF135 may participate in both ABA and ET signaling pathways to regulate the responses to multiple stresses.

【 授权许可】

Unknown