Development of Quality Control Ranges for Biocide Susceptibility Testing

Pathogens

the Biocide Susceptibility Study Group¹  Marita Meurer²  Anissa D. Scholtzek³  Andrea T. Feßler³  Angela R. Schug³  Stefan Schwarz³  John Turnidge⁴ 

[1] ;Institute for Biochemistry, University of Veterinary Medicine Hannover, 30559 Hannover, Germany;Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany;School of Biological Sciences, University of Adelaide, Adelaide, SA 5005, Australia;

关键词: Staphylococcus aureus;    Enterococcus hirae;    Escherichia coli;    Pseudomonas aeruginosa;    benzalkonium chloride;    chlorhexidine;   

DOI  :  10.3390/pathogens11020223

来源: DOAJ

【 摘 要 】

Every laboratory test needs validation by quality controls. For biocide susceptibility testing (BST), neither quality control (QC) strains nor QC ranges applicable to these strains are currently available. As QC strains, four well-defined laboratory reference strains (Staphylococcus aureus ATCC^® 6538, Enterococcus hirae ATCC^® 10541, Escherichia coli ATCC^® 10536 and Pseudomonas aeruginosa ATCC^® 15442), which have been used previously for biocide efficacy testing, were selected. In an interlaboratory trial with eleven participating laboratories, BST QC ranges should be developed for the aforementioned four strains and the four biocides benzalkonium chloride, chlorhexidine, octenidine and polyhexanide. The performance of three different lots of tryptic soy broth was explored using the broth microdilution method and the data were subsequently evaluated using the RangeFinder software. As a result, QC ranges were defined for all reference strain–biocide combinations, except for P. aeruginosa ATCC^® 15442 with the two biocides chlorhexidine and polyhexanide. The development of the latter two QC ranges was not possible, due to the limited solubility of the biocides in the test range required for P. aeruginosa ATCC^® 15442. The newly developed QC ranges comprise three to five dilution steps. The establishment of QC ranges will contribute to the validation of BST in the future.

【 授权许可】

Unknown