Variation, distribution, and diversity of canonical ammonia-oxidizing microorganisms and complete-nitrifying bacteria in highly contaminated ecological restoration regions in the Siding mine area

Ecotoxicology and Environmental Safety

Ying Liang¹  Jiangming Ma¹  Haichun Zhang²  Yuan Liu³  Jie Xu³  Kehui Liu⁴  Jing Zhu⁴  Zhenming Zhou⁵  Yi Li⁵  Fangming Yu⁵ 

[1] College of Environment and Resources, Guangxi Normal University, 541004 Guilin, China;Innovation Institute of Sustainable Development, Guangxi Normal University, 541004 Guilin, China;College of Environment and Resources, Guangxi Normal University, 541004 Guilin, China;College of Life Science, Guangxi Normal University, 541004 Guilin, China;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, China;

关键词: AOA;    AOB;    Comammox;    Mine area;    Soil organic matter;   

DOI  :  

来源: DOAJ

【 摘 要 】

Canonical ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB) and complete-nitrifying bacteria (comammox) exist in a variety of ecosystems. However, little is known about AOA, AOB and comammox or their contributions to nitrification in the soils of heavily degraded and acidic mine regions. In the present study, the activity, richness, diversity and distribution patterns of AOA, AOB and comammox in the Siding mine area were investigated. Nemerow's multifactor pollution index (PN) values indicated that the soil in all three areas in the Siding mine area was highly contaminated by Cd, Pb, Zn, Mn and Cu. The AOA, AOB and comammox amoA gene copy numbers exhibited significant positive correlations with Pb and Zn levels and PN values, which indicated that the populations of AOA, AOB and comammox underwent adaptation and reproduction in response to pollution from multiple metals in the Siding mine area. Among them, the abundance of AOA was the highest, and AOA may survive better than AOB and comammox under such severely pollution-stressed and ammonia-limited conditions. The phyla Thaumarchaeota and Crenarchaeota may play vital roles in the soil ammonia oxidation process. Unlike AOA, AOB may use soil available phosphorus to help them compete for NH3 and other limiting nutrients with AOA and heterotrophs. Moreover, soil organic matter was the main factor influencing the species diversity of AOB, the β-diversity of AOB and comammox, and the community composition of AOA, AOB and comammox. Our research will help to explain the role and importance of AOA, AOB and comammox in the different ecological restoration regions in the Siding mine area.

【 授权许可】

Unknown