A stratification model of hepatocellular carcinoma based on expression profiles of cells in the tumor microenvironment

BMC Cancer

Fengsheng Chen¹  Chunting Zeng²  Zelong Luo³  Jiarong Li³  Linfeng Fan⁴  Chanhua Luo⁵  Xiaowen Huang⁶  Linmeng Zhang⁷  Chen Yang⁷ 

[1] Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University;Cancer Center, Nanfang Hospital, Southern Medical University;Cancer Center, Zhujiang Hospital, Southern Medical University;Digestive system department, The Fifth Affiliated Hospital Sun Yat-sen University;Digestive system department, The Sixth Affiliated Hospital, South China University of Technology;Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Renji Hospital, Shanghai Cancer Institute, Shanghai Institute of Digestive Disease, Shanghai Jiao Tong University School of Medicine;State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine;

关键词: Hepatocellular carcinoma;    Immunotherapy;    Tumor microenvironment;    Tumor treatment;    Stratification model;   

DOI  :  10.1186/s12885-022-09647-5

来源: DOAJ

【 摘 要 】

Abstract Background A malignancy of the liver, hepatocellular carcinoma (HCC) is among the most common and second-leading causes of cancer-related deaths worldwide. A reliable prognosis model for guidance in choosing HCC therapies has yet to be established. Methods A consensus clustering approach was used to determine the number of immune clusters in the Cancer Genome Atlas and Liver Cancer-RIKEN, JP (LIRI_JP) datasets. The differentially expressed genes (DEGs) among these groups were identified based on RNA sequencing data. Then, to identify hub genes among signature genes, a co-expression network was constructed. The prognostic value and clinical characteristics of the immune clusters were also explored. Finally, the potential key genes for the immune clusters were determined. Results After conducting survival and correlation analyses of the DEGs, three immune clusters (C1, C2, and C3) were identified. Patients in C2 showed the longest survival time with the greatest abundance of tumor microenvironment (TME) cell populations. MGene mutations in Ffibroblast growth factor-19 (FGF19) and catenin (cadherin-associated protein),β1(CTNNB1) were mostly observed in C2 and C3, respectively. The signature genes of C1, C2, and C3 were primarily enriched in 5, 23, and 26 pathways, respectively. Conclusions This study sought to construct an immune-stratification model for the prognosis of HCC by dividing the expression profiles of patients from public datasets into three clusters and discovering the unique molecular characteristics of each. This stratification model provides insights into the immune and clinical characteristics of HCC subtypes, which is beneficial for the prognosis of HCC.

【 授权许可】

Unknown