Metabolomics Technologies for the Identification and Quantification of Dietary Phenolic Compound Metabolites: An Overview

Antioxidants

Elvira Escribano-Ferrer¹  Anna Vallverdú-Queralt²  Anallely López-Yerena²  Inés Domínguez-López²  RosaM. Lamuela-Raventós²  Maria Pérez²  Olga Jáuregui³ 

[1] CIBER Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III, 28029 Madrid, Spain;Department of Nutrition, Food Science and Gastronomy XaRTA, Institute of Nutrition and Food Safety (INSA-UB), Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, Spain;Scientific and Technological Center (CCiTUB), University of Barcelona, 08028 Barcelona, Spain;

关键词: phenolic compounds;    metabolites;    sample treatment;    NMR;    LC/MS;    urine;   

DOI  :  10.3390/antiox10060846

来源: DOAJ

【 摘 要 】

In the search for natural products with properties that may protect against or slow down chronic and degenerative diseases (e.g., cancer, and cardiovascular and neurodegenerative conditions), phenolic compounds (PC) with benefits for human health have been identified. The biological effects of PC in vivo depend on their bioavailability, intestinal absorption, metabolism, and interaction with target tissues. The identification of phenolic compounds metabolites (PCM), in biological samples, after food ingestion rich in PC is a first step to understand the overall effect on human health. However, their wide range of physicochemical properties, levels of abundance, and lack of reference standards, renders its identification and quantification a challenging task for existing analytical platforms. The most frequent approaches to metabolomics analysis combine mass spectrometry and NMR, parallel technologies that provide an overview of the metabolome and high-power compound elucidation. In this scenario, the aim of this review is to summarize the pre-analytical separation processes for plasma and urine samples and the technologies applied in quantitative and qualitative analysis of PCM. Additionally, a comparison of targeted and non-targeted approaches is presented, not available in previous reviews, which may be useful for future metabolomics studies of PCM.

【 授权许可】

Unknown