Evolution, Expression Profile, Regulatory Mechanism, and Functional Verification of EBP-Like Gene in Cholesterol Biosynthetic Process in Chickens (Gallus Gallus)

Frontiers in Genetics

Zhang Wang¹  Keren Jiang¹  Yanbin Wang³  Hong Li³  Yadong Tian³  Donghua Li³  Xiaojun Liu³  Zheng Ma⁴ 

[1] College of Animal Science, Henan Agricultural University, Zhengzhou, China;Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, China;International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou, China;School of Life Sciences and Engineering, Foshan University, Foshan, China;

关键词: EBP-like;    liver;    cholesterol synthesis;    chicken;    estrogen;   

DOI  :  10.3389/fgene.2020.587546

来源: DOAJ

【 摘 要 】

The emopamil binding protein (EBP) is an important enzyme participating in the final steps of cholesterol biosynthesis in mammals. A predictive gene EBP-like, which encodes the protein with a high identity to human EBP, was found in chicken genome. No regulatory mechanisms and biological functions of EBP-like have been characterized in chickens. In the present study, the coding sequence of EBP-like was cloned, the phylogenetic trees of EBP/EBP-like were constructed and the genomic synteny of EBP-like was analyzed. The regulatory mechanism of EBP-like were explored with in vivo and in vitro experiments. The biological functions of EBP-like in liver cholesterol biosynthetic were examined by using gain- or loss-of-function strategies. The results showed that chicken EBP-like gene was originated from a common ancestral with Japanese quail EBP gene, and was relatively conservative with EBP gene among different species. The EBP-like gene was highly expressed in liver, its expression level was significantly increased in peak-laying stage, and was upregulated by estrogen. Inhibition of the EBP-like mRNA expression could restrain the expressions of EBP-like downstream genes (SC5D, DHCR24, and DHCR7) in the cholesterol synthetic pathway, therefore downregulate the liver intracellular T-CHO level. In conclusion, as substitute of EBP gene in chickens, EBP-like plays a vital role in the process of chicken liver cholesterol synthesis. This research provides a basis for revealing the molecular regulatory mechanism of cholesterol synthesis in birds, contributes insights into the improvement of the growth and development, laying performance and egg quality in poultry.

【 授权许可】

Unknown