Antibiotic-Resistant Escherichia coli and Sequence Type 131 in Fecal Colonization in Dogs in Taiwan

Microorganisms

Wen-Chien Ko¹  Jiun-Ling Wang¹  Jenn-Wei Chen²  Szu-Min Chang²  Chih-Ming Chen³  Yu-Lung Chiu⁴  HanHsiang Huang⁴  Joy Scaria⁵ 

[1] Department of Internal Medicine, National Cheng Kung University Hospital, Tainan 701, Taiwan;Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan;Department of Nursing, Jenteh Junior College of Medicine, Nursing and Management, Miaoli 356, Taiwan;Department of Veterinary Medicine, National Chiayi University, Chiayi City 60054, Taiwan;Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA;

关键词: dog;    ST131;    ESBL;    fecal colonization;    Escherichia coli;   

DOI  :  10.3390/microorganisms8091439

来源: DOAJ

【 摘 要 】

Background: Most drug-resistant Escherichia coli isolates in dogs come from diseased dogs. Prior to this study, the prevalence and risk factors of fecal carriage drug-resistant E. coli and epidemic clone sequence type (ST) 131 (including subtypes) isolates in dogs were unknown. Methods: Rectal swabs were used for E. coli isolation from 299 non-infectious dogs in a veterinary teaching hospital in Taiwan. Antibiotic resistance and multiplex PCR analyses of E. coli for major STs were performed. Result: There were 43.1% cefazolin-resistant, 22.1% fluoroquinolone-resistant, and 9.4% extended-spectrum beta-lactamase-producing E. coli in our cohort. In the phylogenetic study, B2 was the predominant group (30.1%). The cefazolin-resistant group and ciprofloxacin-resistant group had greater antibiotic exposure in the last 14 days (p < 0.05). The age, sex, and dietary habits of the antibiotic-resistant and -susceptible groups were similar. In the seven isolates of ST131 in fecal colonization, the most predominant subtypes were FimH41 and FimH22. Conclusion: Recent antibiotic exposure was related to the fecal carriage of antibiotic-resistant E. coli isolates. Three major subtypes (FimH41, H22, and H30) of ST131 can thus be found in fecal carriage in dogs in Taiwan.

【 授权许可】

Unknown