Identification of novel neutrophil very long chain plasmalogen molecular species and their myeloperoxidase mediated oxidation products in human sepsis

Redox Biology

Matthew J. Jellinek¹  Carolyn J. Albert¹  Daniel F. Hoft¹  Nuala J. Meyer¹  David A. Ford²  Michael G.S. Shashaty³  Megan P. Kilroy³  Valerio Rasi³  Kaushalya Amunugama³ 

[1] Center for Cardiovascular Research, Saint Louis University School of Medicine, St. Louis, MO, 63104, USA;Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, MO, 63104, USA;Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO, 63104, USA;

关键词: Plasmalogens;    Chlorolipids;    Myeloperoxidase;    Neutrophil activation;    Sepsis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Plasmalogens are a class of phospholipids containing vinyl ether linked aliphatic groups at the sn-1 position. Plasmalogens are known to contain 16- and 18-carbon aliphatic groups at the sn-1 position. Here, we reveal that the human neutrophil plasmenylethanolamine pool uniquely includes molecular species with very long carbon chain (VLC) aliphatic groups, including 20-, 22- and 24-carbon vinyl ether linked aliphatic groups at the sn-1 position. We identified these novel VLC plasmalogen species by electrospray ionization mass spectrometry methods. VLC plasmalogens were only found in the neutrophil plasmenylethanolamine pool. During neutrophil activation, VLC plasmenylethanolamines undergo myeloperoxidase-dependent oxidation to produce VLC 2-chlorofatty aldehyde and its oxidation product, 2-chlorofatty acid (2-ClFA). Furthermore, plasma concentrations of VLC 2-ClFA are elevated in human sepsis. These studies demonstrate for the first time VLC plasmenylethanolamine molecular species, their myeloperoxidase-mediated chlorolipid products and the presence of these chlorolipids in human sepsis.

【 授权许可】

Unknown