A Blue Light-Inducible CRISPR-Cas9 System for Inhibiting Progression of Melanoma Cells

Frontiers in Molecular Biosciences

Jianzhong Zhang¹  Xia Wu²  Bo Yu²  Haiyan Huang² 

[1] Department of Dermatology, Peking University People’s Hospital, Beijing, China;Department of Dermatology, Skin Research Institute of Peking University Shenzhen Hospital, Peking University Shenzhen Hospital, Shenzhen, China;

关键词: melanoma;    light-inducible system;    CRISPR/Cas9;    gene switch;    BRAF V600E;   

DOI  :  10.3389/fmolb.2020.606593

来源: DOAJ

【 摘 要 】

Melanoma is an aggressive skin tumor that shows a high mortality rate and level of metastasis. BRAF gene mutation (BRAF V600E) is directly related to the occurrence of melanoma. In this study, a light-inducible gene expression system was designed to control the Cas9 transcription, which could then cleave the BRAF V600E. To prove the potential utility of this system in melanoma, the physiological function of melanoma cells was tested. It illustrated that the light-induced CRISPR-Cas9 system could inhibit the progression of G361 and A375 cells. Thus, this system may provide a novel therapeutic strategy of melanoma intervention.

【 授权许可】

Unknown