THE PHYSICAL FORM OF CORN INFLUENCES THE RUMEN BACTERIAL BIODIVERSITY – PRELIMINARY RESULTS

Poljoprivreda

Gabriel De La Fuente¹  Franco Tagliapietra²  Giovanni Bittante²  Giulia Rossi³  Stefano Schiavon³  Laura Maccarana⁴  Eric Pinloche⁵  Jamie Newbold⁵ 

[1] Department of Animal Production, University of Lleida, Alcalde Rovira Roure 191, 25198 Lleida, Spain ;Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy ;Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy;Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Legnaro (PD), Italy ;Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, SY23 3AL, United Kingdom ;

关键词: rumen bacterial biodiversity;    diet composition;    physical form;    OTUs;   

DOI  :  10.18047/poljo.21.1.sup.21

来源: DOAJ

【 摘 要 】

The aim of this study was to investigate the rumen bacteria in terms of genetic biodiversity and variation due to different physical form of corn in cow diet. A total of twenty dry cows were fed for 3 months with the same diet, only differed for corn physical form, ten received corn grains, while the other ones received corn flour. To investigate the biodiversity of the bacterial 16S rRNA gene clone library analysis has been conducted and then the sequencing has been carried out using Ion Torrent PGM™ System. Bacterial population was tested using R statistical software. The Kruskal-Wallis one-way analysis of variance (Kruskal-Wallis, 1952) confirmed that the bacterial populations were different when the animals were fed grain compared with flour corn. Both the OUT’s abundance (Operational Taxonomic Unit) and the biodiversity indexes presented a significant difference among the two sample groups, underlining the large changes that take place even with small diet modifications in ruminal environment. There is still the need to deepen how exactly the diet changes the rumen phylogenetic structure and the consequences on bacteria’s activity.

【 授权许可】

Unknown