From Water into Sediment—Tracing Freshwater Cyanobacteria via DNA Analyses

Microorganisms

Achim Brauer¹  Sylvia Pinkerneil¹  Lars Ganzert²  Dirk Wagner²  Ebuka Canisius Nwosu²  Susanne Liebner²  Sizhong Yang²  Elke Dittmann³  Olaf Dellwig⁴  Patricia Roeser⁴ 

[1] GFZ German Research Centre for Geosciences, Section Climate Dynamics and Landscape Evolution, 14473 Potsdam, Germany;GFZ German Research Centre for Geosciences, Section Geomicrobiology, 14473 Potsdam, Germany;Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany;Leibniz Institute for Baltic Sea Research Warnemünde, Marine Geology, 18119 Rostock, Germany;

关键词: Aphanizomenon;    Planktothrix;    Snowella;    cyanobacteria sedimentation;    lake monitoring;    sedimentary ancient DNA;   

DOI  :  10.3390/microorganisms9081778

来源: DOAJ

【 摘 要 】

Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from sediments is crucial, especially in lakes lacking long-term monitoring data. To extend the resolution of sediment record interpretation, we used high-throughput sequencing, amplicon sequence variant (ASV) analysis, and quantitative PCR to compare pelagic cyanobacterial composition to that in sediment traps (collected monthly) and surface sediments in Lake Tiefer See. Cyanobacterial composition, species richness, and evenness was not significantly different among the pelagic depths, sediment traps and surface sediments (p > 0.05), indicating that the cyanobacteria in the sediments reflected the cyanobacterial assemblage in the water column. However, total cyanobacterial abundances (qPCR) decreased from the metalimnion down the water column. The aggregate-forming (Aphanizomenon) and colony-forming taxa (Snowella) showed pronounced sedimentation. In contrast, Planktothrix was only very poorly represented in sediment traps (meta- and hypolimnion) and surface sediments, despite its highest relative abundance at the thermocline (10 m water depth) during periods of lake stratification (May–October). We conclude that this skewed representation in taxonomic abundances reflects taphonomic processes, which should be considered in future DNA-based paleolimnological investigations.

【 授权许可】

Unknown